Recombinant Peptostreptococcus magnus Protein L (r-PPL)

Protein L, derived from Streptococcus Alimentarius, is a protein that can bind to a wide range of immunoglobulins. It only binds to the kappa light chain of the antibody without affecting the antigen-binding site of the antibody. Compared to protein A or protein G, protein L can bind antibodies from various sources and subclasses more broadly.

It does not bind immunoglobulins from cattle, goats, or sheep, and can be used to purify monoclonal antibodies from a culture medium supplemented with BSA or FCS.

The protein L is separated from the surface of the bacterial species Peptostreptococcus magnus and is found to unite with Ig (IgG, IgM, IgA, IgE and IgD) through the interaction of the cadena L, from the cual se sugirió el number. A pesar de esta capacidad de unión de amplio rango con respecto a las clases de Ig, la proteína L no es una proteína de unión a inmunoglobilina universal. La unión de la proteína L a las inmunoglobulinas está restringida a aquellas que contienen cadenas ligeras kappa (es decir, la cadena k del dominio VL).

In humanos y ratones, predominates las cadenas ligeras kappa (k). The remaining immunoglobulinas tienen cadenas ligeras lambda (l). The recombinant protein contains cuatro dominios de unión a immunoglobulina (Ig) (dominios B) of the native protein. Además del anticuerpo, la proteína L también es adecuada para la unión de una amplia gama of fragments of anticuerpos como Fabs, variable fragments of cadena sencilla (scFv) y anticuerpos de dominio (Dabs).

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