Education:
 | PhD, 1981, Free University of Berlin, Germany |
Position:
| Professor and Section Leader Section of Developmental Genetics and Biotechnology University of Vienna, Austria |
Research interests:
| Molecular mechanisms of plant development and of the interaction of plants with their physical and biotic environment. In particular: |
| Signal transduction by protein kinases |
| Pollen development |
| Plant cell cycle |
| Totipotency |
| Pollen transformation and the regeneration of haploid plants from pollen. |
Selected publications:
PAPERS IN INTERNATIONAL, REVIEWED JOURNALS
STÖGER E, FINK C, PFOSSER M, HEBERLE-BORS E (1995) Plant transformation by particle bombardment of embryogenic pollen. Plant Cell Rep 14:273-278.
JONAK C, HEBERLE-BORS E, HIRT H (1995) A cDNA from Medicago sativa encodes a protein homologous to small GTP-binding proteins. Plant Physiol 107:263-264.
EINZENBERGER E, ELLER N, HEBERLE-BORS E, VICENTE O (1995) Isolation and expression during pollen development of a tobacco cDNA clone encoding a protein kinase homologous to shaggy/glycogen synthase kinase-3. Biochem Biophys Acta 1260:315-319.
MITTERMANN I, SWOBODA I, PIERSON E, ELLER N, KRAFT D, VALENTA R, HEBERLE-BORS E (1995) cDNA cloning and characterization of profilin from tobacco (Nicotiana tabacum): increased profilin expression during pollen maturation. Plant Molec Biol: 27:137-146.
ZARSKY V, GARRIDO D, ELLER N, TUPY J, VICENTE O, SCHÖFFL F, HEBERLE-BORS E (1995) The expression of a small heat shock gene is activated during induction of tobacco pollen embryogenesis by starvation. Plant, Cell and Environment 18:139-147.
YLSTRA B, TOURAEV A, BRINKMANN AO, HEBERLE-BORS E, VAN TUNEN AJ (1995) Steroid sex hormones stimulate germination and tube growth of in vitro matured tobacco pollen. Plant Physiol 107:639-643.
SWOBODA I, JILEK A, FERREIRA F, HOFFMANN-SOMMERGRUBER K, SCHEINER O, KRAFT D, BREITENEDER H, PITTENAUER E, SCHMID E, VICENTE O, HEBERLE-BORS E, AHORN H, BREITENBACH M (1995a) Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry and cDNA cloning. J Biol Chem 270:2607-2613.
JONAK C, HEBERLE-BORS E, HIRT H (1995) Inflorescence-specific expression of AtK-1, a novel Arabidopsis thaliana homologue of shaggy/glycogen-synthase kinase-3. Plant Mol Biol 27:217-221.
TOURAEV A, LEZIN F, HEBERLE-BORS E, VICENTE O (1995) Maintenance of gametophytic development after symmetrical division in tobacco microspore culture. Sex Plant Reprod 8:70-76.
LÖSCHENBERGER F, PFOSSER M, HEBERLE-BORS E (1995) Genetic variability for the frequency of doubled haploid green plants is correlated with the ratio of green to albino plants in wheat (Triticum aestivum L.) microspore-derived plants. J Genet & Breed 49:37-44.
SWOBODA I, HA DTC, HEBERLE-BORS E, VICENTE O (1995b) Expression of Bet v1, the major birch pollen allergen, during anther development: an in situ hybridisation study. Protoplasma 187:103-110..
MESKIENE I, BÖGRE L, DAHL M, HEBERLE-BORS E, AMMERER G, HIRT H (1995) Cloning of a potential G1 cyclin of alfalfa by providing pheromone resistance to yeast. The Plant Cell 7:759-771.
GARRIDO D, VICENTE O, HEBERLE-BORS E, RODRIGUEZ-GARCIA MI (1995) Cellular changes during the acquisition of embryogenic potential in isolated pollen of Nicotiana tabacum. Protoplasma 186:220-230.
SWOBODA I, SCHEINER O, HEBERLE-BORS E, VICENTE O (1995c) cDNA cloning and characterization of three birch genes, members of the Bet v 1 gene family of pollen allergens, that encode pathogenesis-related proteins. Plant, Cell and Environment 18:865-874.
WILSON C, ANGLMAYR R, VICENTE O, HEBERLE-BORS E (1995) Molecular cloning, functional expression in Escherichia coli, and characterization of multiple MAP kinases from tobacco. Eur J Biochem 233, 249-257.
PFOSSER M, AMON A, LELLEY T, HEBERLE-BORS E (1995) Evaluation of sensitivity of flow cytometry in detecting DNA amount variation using disomic and ditelosomic wheat/rye addition lines. Cytometry 21: 387-393.
TOURAEV A, FINK CS, STÖGER E, HEBERLE-BORS E (1995) Pollen selection: a transgenic approach. Proc Natl Acad Sci USA 92:12165-12169.
DAHL M, MESKIENE I, BÖGRE L, HA DTC, SWOBODA I, HUBMANN R, HIRT H, HEBERLE-BORS E (1995) The D-type alfalfa cyclin gene cycMs4 complements G1 cyclin deficient yeast and is induced in the G1 phase of the cell cycle. The Plant Cell 7: 1847-1857.
PFOSSER M, AMON A, LAFFERTY J, HEBERLE-BORS E, LELLEY T (1995) The gain or loss of single chromosomes in 6x Triticale is detectable by flow cytometry. J Plant Breed 114:555-557.
SWOBODA I, HOFFMANN-SOMMERGRUBER K, O'RIORDAIN G, SCHEINER O, HEBERLE-BORS E, VICENTE O (1996) Bet v 1 proteins, the major birch pollen allergens and members of a family of conserved pathogenesis-related proteins, are ribonucleases. Physiol Plant 96:433-438.
TOURAEV A, ILHAM A, VICENTE O, HEBERLE-BORS E (1996) Stress-induced microspore embryogenesis in tobacco: an optimized system for molecular studies. Plant Cell Reports 15:561-565.
BÖGRE L, JONAK C, MINK M, MESKIENE I, TRAAS J, HA DTC, SWOBODA I, PLANK C, WAGNER E, HEBERLE-BORS E, HIRT H (1996) Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin. The Plant Cell 8:417-428.
MITTERMANN I, HEBERLE-BORS E, VALENTA R (1996) Molecular characterization of profilin isoforms from tobacco (Nicotiana tabacum) pollen. Sex Plant Reprod 9:133-139.
TOURAEV A, INDRIANTO A, WRATSCHKO I, VICENTE O, HEBERLE-BORS E (1996) Starvation and heat-shock-induced in vitro microspore embryogenesis in wheat (Triticum aestivum L.). Sex Plant Reprod 9:209-215.
TOURAEV A, PFOSSER M, VICENTE O, HEBERLE-BORS E (1996) Stress as the major signal controlling the developmental fate of tobacco microspores: towards a unified model of induction of microspore/pollen embryogenesis. Planta 200:144-152.
BÖGRE L, LIGTERINK W, HEBERLE-BORS E, HIRT H (1996) Mechanosensors in plants. Nature 383:489-490
BÖGRE L, LIGTERINK W, MESKIENE I, BAKER PJ; HEBERLE-BORS E, HUSKISSON N, HIRT H (1997) Wound signalling of plants: activation of a specific MAP kinase pathway. Plant Cell 9:75-83.
BÖGRE L, ZWERGER K, MESKIENE I, BINAROVA P, CSIZMADIA V, PLANCK C, WAGNER E, HIRT H, HEBERLE-BORS (1997) The cdc2Ms kinase is differently regulated in the cytoplasm and in the nucleus. Plant Physiology 113:841-852.
WILSON C, HEBERLE-BORS E, VICENTE O (1997) A conserved motif at the carboxy terminus of MAP kinases. J. Mol. Evol. 44:683-685.
WILSON C, VORONIN V, TOURAEV A, VICENTE O, HEBERLE-BORS E (1997) A developmentally regulated MAP kinase activated by hydration in tobacco pollen. The Plant Cell 9:2093-2100.
TOURAEV A, STÖGER E, VORONIN V, HEBERLE-BORS E (1997) Plant male germ line transformation. The Plant Journal 12 (4), 949-956.
WILSON C; PFOSSER M; JONAK C; HIRT H; HEBERLE-BORS E; VICENTE O (1998) Evidence for the activation of a MAP kinase upon phosphate-induced cell cycle re-entry in tobacco cells. Physiol Plant. 102:532-538.
MITTERMANN, I., FETROW, J., SCHAAK, D., ALMO, S. C., KRAFT, D., HEBERLE-BORS E., VALENTA R. (1998) Oligomerization of profilins from birch, man and yeast. Profilin, a ligand for itself?. Sex Plant Reprod., 11:183-191.
Calderini O, Bögre L, Vicente O, Binarova P, Heberle-Bors E, Wilson C (1998) A cell cycle regulated MAP kinase with a possible role in cytokinesis in tobacco cells. J. Cell Sci. 111, 3091-3100.
BINAROVA P, DOLEZEL J, HEBERLE-BORS E, STRNAD M, BÖGRE L (1998) Treatment of Vicia faba root tip cells with specific inhibitors to cyclin-dependent kinases leads to abnormal spindle formation. Plant J 16, 697-707.
BÖGRE L, CALDERINI O, BINAROVA P, MATTAUCH M, TILL S, KIEGERL S, JONAK C, POLLASCHEK C, BARKER P, HUSKISSON NS, HIRT H, HEBERLE-BORS E ( 1998) A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division. The Plant Cell 11:101-113.
INDRIANTO A, HEBERLE-BORS, E, TOURAEV A (1999) Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores. Plant Science 143, 71-79.
PRESTAMO P, TESTILLANO PS, VICENTE O, GONZALEZ-MELENDI P, CORONADO MJ, WILSON C, HEBERLE-BORS E, RISUENO MC (1999) Ultrastructural distribution of a MAP kinase homologue and their transcripts in quiescent and cycling plant cells, and in pollen grains. J Cell Sci 112, 1065-1076.
HOEFER M, TOURAEV A, HEBERLE-BORS E (1999) Induction of embryogenesis from isolated apple microspores. Plant Cell Rep. 18:1012-1017.
OTTENSCHLÄGER I, BARINOVA I, VORONIN V, DAHL M, HEBERLE-BORS E, TOURAEV A (1999) Green Fluorescent Protein (GFP) as a marker during pollen development. Transgenic Research 8, 279-294.
CAZALE AC, DROLLARD MJ, WILSON C, HEBERLE-BORS E, BARBIER-BRYGOO H, LAURIERE C (1999) MAP kinase activation by hypoosmotic stress on tobacco cell suspensions:towards the oxidative burst response. Plant J 19, 297-308.
FUJIKURA Y, DOLEZEL J, CIHALIKOVA J, BÖGRE L, HEBERLE-BORS, E, HIRT, H, BINAROVA P (1999) Vicia faba germination: synchronized cell growth and localisation of nucleolin and alpha-tubulin. Seed Science Research 9: 297-304.
INDRIANTO A, BARINOVA A, TOURAEV A, HEBERLE-BORS E (2001) Tracking individual wheat microspores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta 212: 163-174.
VORONIN, V., TOURAEV, A., KIEFT, H., VAN LAMMEREN, A., HEBERLE-BORS, E., WILSON, C. (2001) Temporal and tissue-specific expression of the tobacco ntf4 MAP kinase. Plant Mol. Biol. (in press)
CALDERINI, O., GLAB, N., BERGOUNIOUX, C., HEBERLE-BORS, E., WILSON C. (2001) A novel tobacco MAP kinase kinase, NtMEK1, activates the cell cycle-regulated p43Ntf6 MAP kinase. J. Biol. Chem 276, 18139-18145.
REVIEW ARTICLES
HEBERLE-BORS E (1995) Transformation via microspore embryogenesis. Rice Biotechnology Quaterly 23:17-18.
TOURAEV A, VICENTE O, HEBERLE-BORS E (1997) Induction of microspore embryogenesis by stress. Trends in Plant Sci. 2:297-302.
HEBERLE-BORS E; DE VRIES S (1997) Le développment embryonnaire des végétaux. Biofutur 172, 38-42.
HEBERLE-BORS E (1998) Experimental control of pollen development. In: Androgenesis and Haploid Plants (CHYPEAU Y; CABOCHE M; HENRY Y, eds.) INRA Edition, Springer Berlin Heidelberg INRA Paris, pp. 38-53.
HIRT H, BÖGRE L, MESKIENE I, DAHL M, ZWERGER K, HEBERLE-BORS E (1998) Functional isolation and analysis of plant cell cycle genes in yeast. In Molecular and Cellular Biology of the Plant Cell Cycle (D. Francis, D. Dudits, D. Inzé, eds) Portland Press Ltd, London, pp 129-143.
Heberle-Bors E (1998) Stress treatments for turning immature pollen into plants. In: PBI Bulletin (The Plant Biotechnology Institute, National Research Counsil Canada): Plants from pollen: biotech implications, p. 9-10.
Heberle-Bors E (1999) Microspore culture, totipotency, and doubled haploids in plant breeding. In Vitro Cell Dev. Biol. - Plant 35: 165-167.
Touraev, A. and Shamina, Z. (1999). Cotton pollen culture in vitro (a review). In. " Biotechnology in Agriculture and Forestry, ed. Y.P.S.Bajaj. (Springer Int.).
Wilson C, Heberle-Bors E (2000) MAP kinases in pollen. In: MAP kinases in plant signal transduction (H. Hirt, ed.), Springer-Verlag, Berlin Heidelberg New York, pp. 39-51.
Jérôme Joubès, Christian Chevalier, Denes Dudits, Erwin Heberle-Bors, Dirk Inzé, Masaaki Umeda, and Jean-Pierre Renaudin. CDK-related protein kinases in plants. Plant Mol Biol. 43:607-620.
Bögre L, Meskiene I, Heberle-Bors E, Hirt H (2000) Stressing the role of MAP kinases in mitogenic stimulation. Plant Mol Biol 43:705-718.
Touraev A, Tashpulatov A, Indrianto A Katholnigg H, Barinova I, Voronin V, Akimcheva S, Ribarits A. Zhexembekova M, and Heberle-Bors E (2000) Fundamental aspects of microspore embryogenesis induction. In "Biotechnological Approaches for Utilization of Gametic Cells - COST824 (B. Bohanec, Ed.), pp. 205-214, Brussels.
Heberle-Bors E (2001) Cyclin-dependent protein kinases, mitogen-activated protein kinases and the plant cell cycle. Current Science 80: 225-232.
Touraev, A., Pfosser, M., Heberle-Bors, E. (2001). Microspores- a haploid multipurpose cell. In: "Advances in Botanical Research, in press
Heberle-Bors E, Calderini O, Voronin V, and Wilson C (2001) Signal transduction by mitogen-activated protein kinases (MAPKs). IN: Signal transduction in plants (Sopory S., ed.) Kluwer Academic/Plenum Press, in press.
Heberle-Bors E, Voronin V, Touraev A, Sanchez Testillano P, Risueño MC, & Wilson C. MAP kinase signaling during pollen development. Sex Plant Reprod., in press.
Patents
HEBERLE-BORS, E., BENITO MORENO, R.M., ALWEN, A. (1988) Verfahren zum Gentransfer in
Pflanzen. Patentschrift des Patentamts der Republik Österreich Nr. 388 384 B. Europäische
Patentanmeldung, Veröffentlichungsnummer: 0 301 316 A2.
HEBERLE-BORS E, BENITO MORENO RM, ALWEN A, TOURAJEW A, STÖGER EM (1998) Method for producing seeds and plants thereof involving an in vitro step.
Assigne: MOGEN International nv, Netherlands. United States Patent No. 5,840,557, Nov. 24, 1998.
HEBERLE-BORS E; BÖGRE L, WEINGARTNER M (1999) Method modifying plant metabolism, morphology and development. United States Patent Application, march 1999.
Assigne: CropDesign CV, Belgium.