Chun-Ming's Favorite Molecular Biology Protocols

The protocols listed here either designed by myself or modified from other people's published method, but all of them have been tested on my bench and proved work very well in our lab condition.

• Plasmid or Cosmid DNA Miniprep
• Phage DNA Extraction
• BAC DNA Extraction
• Preparation of ultracompetent E. coli cells (best of all!)
• 5 minute transformation method (works!)
• Cloning PCR product to pCRScript vector
• One tube PCR cloning method without using expensive kits
• Genomic DNA extraction from Arabidopsis (CTAB method)
• RNA extraction from Arabidopsis
• Differential display
• TAIL-PCR
for gene cloning from T-DNA tagged mutants (Feldmann's lines only)
• Non-radioactive in situ hybridization
• How to prepare YAC end probes
• How to prepare BAC end probes

Other Good Protocols

• My favorate protocols from other labs
• Arabidopsis protocols
• Arabidopsis transformation

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Comments or Questions? Send to me at:chun-mingliu@plant.wag-ur.nl


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