Chun-Ming Liu's Favorite Molecular Biology Protocols

 

The protocols listed here either designed by myself or modified from other people's published method, but all of them have been tested on my bench and proved work very well in our lab condition.
bulletPlasmid or Cosmid DNA Miniprep
bulletPhage DNA Extraction
bulletPreparation of ultracompetent E. coli cells  
bullet5 minute transformation method  
bulletCloning PCR product to pCRScript vector
bulletOne tube PCR cloning method without using expensive kits
bulletGenomic DNA extraction from Arabidopsis (CTAB method)
bulletRNA extraction from Arabidopsis
bulletDifferential display
bulletTAIL-PCR for gene cloning from T-DNA tagged mutants

Other Good Protocols

bulletProtocol-on-line

Comments or Questions? Send to me at:chunming.liu@wur.nl

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