The cytokinesis-defective (cyt) mutants of pea


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The pictures above show how the cyt mutant embryo and seeds look like. The pea embryo at the top-left is a wild-type, and the one at the top-right is a homozygous cyt mutant. The bottom picture shows an opened pea pod from a heterozygous plant. The segragation of this gene produces 25% lethal seeds and 75% wildtypes. These two red arrowheads indicate the seeds containing mutant embryo, in comtrast to the rests containing wild-type embryos.

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These two photos were taken from a cyd embryo at different section planes showing the arrested cell plate formation. As shown in Figure B that the cell plate (P2) is still expanding at its both ends, and the same cell plate in Figure A is arrested at one end (indicated by an arrow). These photos also show that cell wall stubs floating in the middle of the cell (without connecting mother wall, S1) is actually connected to the mother wall at the section plane B. This is consistant with the conclusion that a cell plate has attached to the mother walls before becoming stable.


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