Ref: Sabatini, S. et al., Cell 99, 463-472.
This protocol can be used for clearing intact non-ovule materials of arabidopsis, which can then be observed under Normarski optics. This is an efficient way to analyse root, seedling even flower development without sectioning. This protocol could also be used for clearing GUS stained material, after chlorophyll is removed by 70% ethanol.
|80g Chloral hydrate (Sigma, C8383)|
|10 ml 100% Glycerol (Sigma, G6279)|
|Dissolve by stirring for 1 hr.|